Peptide reconstitution is the process of dissolving a lyophilised (freeze-dried) peptide powder in bacteriostatic water (BAC water) to produce a stable solution. This peptide reconstitution calculator determines the correct BAC water volume to add and the exact draw volume per dose — including the corresponding insulin-syringe unit markings for U-100 syringes. It is designed for researchers working with compounds such as BPC-157, TB-500, Ipamorelin, and other research peptides.

Step 1 — Add bacteriostatic water slowly. Draw the required volume of BAC water into an insulin syringe. Insert the needle through the rubber stopper and direct the liquid down the inside wall of the vial — not directly onto the powder. This prevents foaming and mechanical stress on the peptide structure.

Step 2 — Swirl, do not shake. Gently swirl the vial in a circular motion until the powder is fully dissolved. Shaking creates air bubbles and mechanical agitation that can degrade sensitive peptide bonds.

Step 3 — Store reconstituted peptides refrigerated. Once reconstituted, peptide solutions should be kept at 2–8°C and away from direct light. Most reconstituted peptides remain stable for 4–6 weeks under refrigeration when prepared with bacteriostatic water.

Step 4 — Draw your calculated dose. Using the volume shown above, draw the required number of insulin-syringe units. The calculator automatically converts your target dose into millilitres and U-100 unit markings at your chosen concentration, and warns if the draw volume exceeds your syringe capacity.

For peptide-specific reconstitution notes, storage temperatures, and step-by-step photographs, see our full Peptide Reconstitution & Storage Guide. Unfamiliar with a term? Browse the Peptide Glossary.