Peptide Reconstitution & Storage Guide
A step-by-step protocol for reconstituting lyophilised research peptides, plus storage temperature guidelines and shelf-life information for all Nexyra Lab products.
Step-by-Step Reconstitution Protocol
Prepare your environment
Work in a clean, sterile environment. Wash hands thoroughly. Use a laminar flow hood if available. Ensure all equipment is sterile.
Allow the vial to equilibrate
Remove the peptide vial from cold storage and allow it to come to room temperature for 15-30 minutes before opening. This prevents condensation from contaminating the powder.
Calculate the BAC water volume
Use the Nexyra Lab Peptide Calculator to determine the exact volume of bacteriostatic water (BAC water) required for your desired concentration. Note this figure before proceeding.
Draw the BAC water
Using a fresh sterile syringe, draw the calculated volume of BAC water from a fresh BAC water vial. A 1mL insulin syringe provides the greatest precision for small volumes.
Inject slowly down the side of the vial
Remove the vial cap and swab the rubber stopper with an alcohol wipe. Insert the syringe needle and inject the BAC water slowly down the inside wall of the glass vial — not directly onto the powder. This preserves peptide integrity.
Gently swirl until dissolved
Gently roll or swirl the vial between your palms until the powder is completely dissolved. The solution should become clear. Do not shake vigorously or vortex — this causes foaming and may denature the peptide.
Inspect the solution
The reconstituted solution should be clear and colourless (some peptides like GHK-Cu may have a slight blue tint due to copper). If particulates remain, allow 5 additional minutes of gentle swirling.
Label and store
Label the vial with the peptide name, concentration, date of reconstitution, and expiry (typically 30 days). Store reconstituted peptides at 4°C (refrigerator, not freezer). Keep away from light.
BAC Water vs Sterile Water
BAC Water (Recommended)
- ✅ Contains 0.9% benzyl alcohol (bacteriostatic)
- ✅ Preserves solution for up to 30 days at 4°C
- ✅ Standard for multi-use research vials
- ✅ Compatible with the majority of research peptides
Sterile Water
- ⚠️ No bacteriostatic agent
- ⚠️ Solution should be used within 24 hours
- ✅ Suitable for single-use research applications
- ✅ May be preferred for pH-sensitive compounds
Storage Reference — Nexyra Lab Products
| Peptide | Temperature | Shelf Life |
|---|---|---|
| BPC-157 | -20°C (lyophilised) / 4°C (reconstituted) | Lyophilised: 24 months. Reconstituted: 30 days |
| TB-500 | -20°C (lyophilised) / 4°C (reconstituted) | Lyophilised: 24 months. Reconstituted: 30 days |
| Retatrutide | -20°C (lyophilised) / 4°C (reconstituted) | Lyophilised: 24 months. Reconstituted: 21 days |
| Ipamorelin | -20°C (lyophilised) / 4°C (reconstituted) | Lyophilised: 24 months. Reconstituted: 30 days |
| GHK-Cu | -20°C (lyophilised) / 4°C (reconstituted) | Lyophilised: 24 months. Reconstituted: 30 days |
| NAD+ | -20°C dry / 4°C solution | Powder: 18 months. Solution: 14 days |
| Semax / Selank | -20°C (lyophilised) / 4°C (reconstituted) | Lyophilised: 18 months. Reconstituted: 21 days |
Shelf life is approximate and may vary by batch. Always refer to the COA for batch-specific guidance.
Required Equipment
Reconstitution FAQs
What is the difference between BAC water and sterile water? +
Bacteriostatic water contains 0.9% benzyl alcohol which prevents bacterial growth. This preserves the reconstituted peptide solution for up to 30 days at 4°C. Sterile water has no antimicrobial agent — peptides reconstituted with sterile water should be used within 24 hours or immediately.
Can I use saline solution to reconstitute peptides? +
For most research applications, bacteriostatic water is preferred. Saline (0.9% NaCl) can be used but may affect solubility of some peptides. Consult your specific research protocol.
Why should I inject BAC water down the side of the vial? +
Injecting directly onto the lyophilised peptide powder can damage the fragile peptide structure through mechanical stress. Letting the water run slowly down the glass minimises this disruption.
Can I freeze reconstituted peptides? +
It is generally not recommended to freeze reconstituted peptide solutions as freeze-thaw cycles can degrade peptide integrity. Lyophilised (powder) form should be kept frozen; reconstituted form refrigerated at 4°C.
How do I know how much BAC water to use? +
Use our Peptide Calculator to determine the exact BAC water volume for your desired concentration based on your vial size.